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Effects of Syn on anthocyanin accumulation in VR cells. ( a ) Effects of Syn at concentrations of 0, 0.01, 0.1, 1, 5, and 10 mM on anthocyanin accumulation. Different letters (a,b) above the bar graphs indicate statistically significant differences (Tukey, p < 0.01 or 0.05). ( b ) Effects of Phe, L-Tyro, Tyra, Oct, and Syn (each 5 mM) on anthocyanin accumulation. * and ** indicate significant differences at p < 0.05 and 0.01, respectively, relative to control <t>(Dunnett).</t> VR cells were cultured at 27 °C, 54.2 μmol m −2 s −1 /16 h/day for 7 days. Data are shown as means ± S.E. for three biological replicates ( n = 3).
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Effects of Syn on anthocyanin accumulation in VR cells. ( a ) Effects of Syn at concentrations of 0, 0.01, 0.1, 1, 5, and 10 mM on anthocyanin accumulation. Different letters (a,b) above the bar graphs indicate statistically significant differences (Tukey, p < 0.01 or 0.05). ( b ) Effects of Phe, L-Tyro, Tyra, Oct, and Syn (each 5 mM) on anthocyanin accumulation. * and ** indicate significant differences at p < 0.05 and 0.01, respectively, relative to control (Dunnett). VR cells were cultured at 27 °C, 54.2 μmol m −2 s −1 /16 h/day for 7 days. Data are shown as means ± S.E. for three biological replicates ( n = 3).

Journal: International Journal of Molecular Sciences

Article Title: Application of Synephrine to Grape Increases Anthocyanin via Production of Hydrogen Peroxide, Not Phytohormones

doi: 10.3390/ijms25115912

Figure Lengend Snippet: Effects of Syn on anthocyanin accumulation in VR cells. ( a ) Effects of Syn at concentrations of 0, 0.01, 0.1, 1, 5, and 10 mM on anthocyanin accumulation. Different letters (a,b) above the bar graphs indicate statistically significant differences (Tukey, p < 0.01 or 0.05). ( b ) Effects of Phe, L-Tyro, Tyra, Oct, and Syn (each 5 mM) on anthocyanin accumulation. * and ** indicate significant differences at p < 0.05 and 0.01, respectively, relative to control (Dunnett). VR cells were cultured at 27 °C, 54.2 μmol m −2 s −1 /16 h/day for 7 days. Data are shown as means ± S.E. for three biological replicates ( n = 3).

Article Snippet: 3.20. (Social Survey Research Information, Tokyo, Japan) with the Student’s t -test, Tukey test, or Dunnett test.

Techniques: Control, Cell Culture

Effects of Syn and H 2 O 2 treatments on VR cells and berries. Expression levels of H 2 O 2 -responsive gene Chit4 ( a ) and anthocyanin-biosynthesis-related genes mybA1 ( b ) and UFGT ( c ). Anthocyanin content in Syn- and H 2 O 2 -treated VR cells ( d ). Anthocyanin content in Syn- and H 2 O 2 -treated berry skin ( e ). VR cells were cultured in a medium containing 5 mM Syn and 10 mM H 2 O 2 for 4 days (for gene expression levels) or 7 days (for anthocyanin content) at 27 °C, 54.2 μmol m −2 s −1 /16 h/day. Berries were harvested 9 days after treatment with 1 mM Syn and 300 mM H 2 O 2 . Gene expression levels were estimated by real-time RT-PCR. Data are expression levels relative to actin. Data are means ± S.E. for four biological replicates ( n = 4). * and ** indicate significant differences at p < 0.05 and 0.01, respectively (Dunnett test).

Journal: International Journal of Molecular Sciences

Article Title: Application of Synephrine to Grape Increases Anthocyanin via Production of Hydrogen Peroxide, Not Phytohormones

doi: 10.3390/ijms25115912

Figure Lengend Snippet: Effects of Syn and H 2 O 2 treatments on VR cells and berries. Expression levels of H 2 O 2 -responsive gene Chit4 ( a ) and anthocyanin-biosynthesis-related genes mybA1 ( b ) and UFGT ( c ). Anthocyanin content in Syn- and H 2 O 2 -treated VR cells ( d ). Anthocyanin content in Syn- and H 2 O 2 -treated berry skin ( e ). VR cells were cultured in a medium containing 5 mM Syn and 10 mM H 2 O 2 for 4 days (for gene expression levels) or 7 days (for anthocyanin content) at 27 °C, 54.2 μmol m −2 s −1 /16 h/day. Berries were harvested 9 days after treatment with 1 mM Syn and 300 mM H 2 O 2 . Gene expression levels were estimated by real-time RT-PCR. Data are expression levels relative to actin. Data are means ± S.E. for four biological replicates ( n = 4). * and ** indicate significant differences at p < 0.05 and 0.01, respectively (Dunnett test).

Article Snippet: 3.20. (Social Survey Research Information, Tokyo, Japan) with the Student’s t -test, Tukey test, or Dunnett test.

Techniques: Expressing, Cell Culture, Gene Expression, Quantitative RT-PCR